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Bacteria of Public Health Significance on Fruit and Vegetables Sold Across Markets in Makurdi, Nigeria
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Volume 9, 2021
Issue 1 (March)
Pages: 10-15   |   Vol. 9, No. 1, March 2021   |   Follow on         
Paper in PDF Downloads: 6   Since Mar. 29, 2021 Views: 70   Since Mar. 29, 2021
Iveren Winifred Nyinoh, Department of Biological Sciences, Faculty of Science, Benue State University, Makurdi, Nigeria.
Ooja Bob-Echikwonye, Department of Epidemiology and Community Health, Benue State University Teaching Hospital, Makurdi, Nigeria.
Ikechukwu Elijah Edeh, Department of Biological Sciences, Faculty of Science, Benue State University, Makurdi, Nigeria.
Fruits and vegetables are composed of vitamins, minerals, and phytochemicals and are globally recommended as part of a healthy diet. But despite their immense nutritional benefits, their surfaces can become contaminated with pathogenic microbes leading to illness or death. We examined the surfaces of fresh garden egg (Solanum aethiopicum), carrot (Daucus carota), mangoes (Mangifera indica), and oranges (Citrus sinensis), purchased from the four major fruit markets in Makurdi (Railway, Wurukum, Wadata, and High-level) for bacteria of public health significance and also estimated the total aerobic plate count (TAPC). Samples were tested for the presence of bacteria by culturing on selected media. Characterization of the isolates was achieved using biochemical tests, Gram staining, and microscopy. Six different bacteria were detected -Pseudomonas spp., Staphylococcus aureus, Klebsiella spp., Escherichia coli, Salmonella spp., and Streptococcus spp., in the range 6.25×106 to 10.2×106 cfu/g in the sampled produce. Among the sampled markets, the fruits and vegetable purchased from High-level market revealed the highest contamination. Carrots purchased from High-level had the highest TAPC of 12.2 × 106 cfu/g, mangoes from Wadata market (9.0 × 106 cfu/g), while garden eggs and oranges also from High-level market showed 8.4 × 106 and 7.6 × 106 cfu/g respectively. At 36.6%, carrots had the highest percentage contamination, followed by mangoes (23.3%), while oranges and garden eggs were the least contaminated at 20% each. There are public health and hygiene risks regarding the fruits and vegetables sold in markets in Makurdi. Urgent steps to address these public health concerns are required.
Food Microbiology, Bacterial Contamination, Fresh Produce, Food Safety, Fresh Fruits and Vegetables, Nigeria
Berger, C. N., Sodha, S. V., Shaw, R. K., Griffin, P. M., Pink, D., Hand, P., and Frankel, G. (2010) Fresh fruit and vegetables as vehicles for the transmission of human pathogens. Environmental Microbiology, 12 (9), 2385-2397.
Machado-Moreira, B., Richards, K., Brennan, F., Abram, F., and Burgess, C. M. (2019) Microbial Contamination of Fresh Produce: What, Where, and How? Comprehensive Reviews in Food Science and Food Safety, 18 (6), 1727-1750.
Slavin, J. L and Lloyd, B. (2012). Health Benefits of Fruits and Vegetables. Advances in Nutrition, 3 (4), 506-16. doi: 10.3945/an.112.002154.
Hung, H. C., Joshipura, K. J., Jiang, R., Hu, F. B., Hunter, D., Smith-Warner, S. A., Colditz, G. A., Rosner, B., Spiegelman, D., and Willett, W. C. (2004) Fruit and vegetable intake and risk of major chronic disease. Journal of the National Cancer Institute, 3, 96 (21): 1577-84.
Oyebode, O., Gordon-Dseagu, V., Walker, A and Mindell J. S. (2014) Fruit and vegetable consumption and all-cause, cancer and CVD mortality: analysis of Health Survey for England data. Journal of Epidemiology and Community Health, 68 (9): 856-62. doi: 10.1136/jech-2013-203500.
Pomerleau, J., Lock, K and McKee, M. (2006) The burden of cardiovascular disease and cancer attributable to low fruit and vegetable intake in the European Union: differences between old and new member states. Public Health Nutrition, 9 (5): 575-83.
Van’t Veer, P, Jansen, M. C., Klerk, M and Kok, F. J. (2000) Fruits and vegetables in the prevention of cancer and cardiovascular disease. Public Health Nutrition, 3 (1): 103-7.
Wang, X., Ouyang, Y., Liu, J., Zhu, M., Zhao, G., Bao, W and Hu, F. B. (2014). Fruit and vegetable consumption and mortality from all causes, cardiovascular disease, and cancer: systematic review and dose response meta-analysis of prospective cohort studies. British Medical Journal, 29, 349: g4490. doi: 10.1136/bmj.g4490.
Carter, P., Gray, L. J., Troughton, J., Khunti, K and Davies, M. J. (2010). Fruit and vegetable intake and incidence of type 2 diabetes mellitus: systematic review and meta-analysis. British Medical Journal, 341, c4229. doi: 10.1136/bmj.c4229.
Muraki, I., Imamura, F., Manson, J. E., Hu, F. B., Willett, W. C., van Dam, R. M and Sun, Q. (2013) Fruit consumption and risk of type 2 diabetes: results from three prospective longitudinal cohort studies. British Medical Journal, 28, 347: f5001. doi: 10.1136/bmj.f5001.
Betts, R. (2014) Microbial update: Fruit and salad. International Food Hygiene, 25 (3), 9-12.
Food and Agricultural Organization (FAO, 2001). Food-based dietary guidelines –Nigeria. http://www.fao.org/nutrition/education/food-dietary-guidelines/regions/countries/Nigeria/en.
DiSogra, L and Taccone, F. (2003) 5 A Day For Better Health Program USA. World Health Organization. https://www.who.int/dietphysicalactivity/media/en/gs_fv_ppt_lorelei.pdf.
Foerster, S. B., Kizer, K. W., Disogra, L. K., Bal, D. G., Krieg, B. F and Bunch, K. L. (1995) California's "5 a day--for better health!" campaign: an innovative population-based effort to effect large-scale dietary change. American Journal of Preventive Medicine, 11 (2), 124-31.
World Health Organization (2019). Fact sheet on Food Safety. Available Online https://www.who.int/en/news-room/fact-sheets/detail/food-safety.
Fung, F., Wang, H-S., and Sure Menon, S. (2018) Food safety in the 21st century. Biomedical Journal, 41 (2), 88-95. doi: 10.1016/j.bj.2018.03.003.
Hald, T., Aspinall, W., Devleesschauwer, B., Cooke, R., Corrigan, T and Havelaar, A. H. (2016) World Health Organization Estimates of the Relative Contributions of Food to the Burden of Disease Due to Selected Foodborne Hazards: A Structured Expert Elicitation. PLoS One, 19, 11 (1), e0145839. doi: 10.1371/journal.pone.0145839.
Ehimemen, N. E., Mukhtar, M. F., Salisu, N. (2019) Prevalence of bacterial loads on some fruits and vegetables sold in kaduna central market, Northwestern Nigeria. Journal of Applied Sciences, 19, 1: 20-4. DOI: 10.3923/jas.2019.20.24.
Adeleke, M. A., Hassan, A. O., Ayepola, T. T., et al (2012). Public health risks associated with apples and carrots sold in major markets in Osogbo, Southwest Nigeria. Journal of Toxicology and Environmental Health Sciences, 4, 140-4. DOI: 10.5897/jtehs12.019.
Eni, A. O., Oluwawemitan, I. A, Oranusi, U. S. (2010) Microbial quality of fruits and vegetables sold in Sango Ota, Nigeria. African Journal of Food Science, 4, 291-6.
Imafidor, H. O., Obemeata, O., Iris, O. (2018) Bacteriological assessment of lettuce vended in Benin City Edo State. International Journal of Environmental and Agriculture Research, 4, 7-13.
Aguoru, C. U., Maaji, S and Olasan, J. O. (2015) Bacteria Contaminants on Surfaces of Some Edible Fruits Sold in Makurdi Metropolis, Benue State, Nigeria. International Journal of Current Microbiology and Applied Sciences, 4 (6), 334-340.
Obetta, S. E., Nwakonobi, T. U., Adikwu, O. A (2011) Microbial effects on selected stored fruits and vegetables under ambient conditions in Makurdi, Benue State, Nigeria. Research Journal of Applied Sciences, Engineering and Technology, 3, 393-8.
Feng, P., Weagant, S. D., Grant, M. A., Burkhardt, W. (2018) Enumeration of Escherichia coli and the Coliform Bacteria. US Food and Drug Administration. Available at https://www.fda.gov/food/laboratory-methods-food/.
Smith, A. C and Hussey, M. A. (2005) Gram stain protocols. American Society for Microbiology. Available Online at https://www.asmscience.org/content/education/protocol/protocol.2886.
Bisen, P. S. (2010) Laboratory Practicals in Applied Life Sciences. London: CRC Press Taylor and Francis group. ISBN 13: 978 – 1- 4665- 5314- 9, pp 708-709.
MacWilliams, M. P. (2009) Indole test protocol.
ASM Science. Available Online at https://www.asmscience.org/content/education/protocol/protocol.3202.
Reiner, K. (2010) Catalase test protocol. ASM. Available Online at https://www.asmscience.org/content/education/protocol/protocol.3226.
Lowy, F. D. (1998) Staphylococcus aureus infections. New England Journal of Medicine, 20, 339 (8), 520-32.
Podschun, R and Ullmann, U. (1998) Klebsiella spp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors. Clinical Microbiology Review, 11 (4), 589–603.
Haenni, M., Lupo, A., Madec, J. Y. (2018) Antimicrobial Resistance in Streptococcus spp. Microbiology spectrum 6 (2). doi: 10.1128/microbiolspec.ARBA-0008-2017.
Morris, J. (2013) Streptococcal Disease In Foodborne Infections and Intoxications. Fourth edition, Academic Press ISBN 978-0-12-416041-5 Pages 223-227.
Akoachere, J. T. K., Tatsinkou, B. F and Nkengfack, J. M. (2018) Bacterial and parasitic contaminants of salad vegetables sold in markets in Fako Division, Cameroon and evaluation of hygiene and handling practices of vendors. BMC Research Notes 6, 11 (1), 100. doi: 10.1186/s13104-018-3175-2.
James, J. B and Ngarmsak, T. (2010) Processing of fresh-cut tropical fruits and vegetables: A TECHNICAL GUIDE. Food and Agriculture Organization of the United Nations Regional Office for Asia and the Pacific Bangkok. ISBN 978-92-5-106712-3.
Beaulieu, J. C., and Gorny, J. L., (2002) Fresh-cut fruits. In K. C. Gross, C. Y. Wang & M. Saltveit, eds. The commercial storage of fruits, vegetables, and florist and nursery crops. USDA. Available at http://www.ba.ars.usda.gov/hb66/index:html.
Ukuku, D. O. and Sapers, G. M. (2001) Effect of sanitizer treatments on Salmonella Stanley attached to the surface of cantaloupe and cell transfer to fresh-cut tissues during cutting practices. Journal of Food Protection 64 (9), 1286-91.
Shiroodi, S. G and Mahmoudreza Ovissipou, M. (2018) Post harvest disinfection of Fruits and vegetables. Edited by Mohammed Wasim Siddiqui Pages 67-89. 1st Edition Academic Press ISBN 978-0-12-812698-1.
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