Welcome to Open Science
Contact Us
Home Books Journals Submission Open Science Join Us News Unsubscribe Page
Uptake and Transport of Manganese Ion in Relation to the Positions of a Root for Pea Plant Observed by Micro-magnetic Resonance Imaging
Current Issue
Volume 6, 2018
Issue 1 (February)
Pages: 8-13   |   Vol. 6, No. 1, February 2018   |   Follow on         
Paper in PDF Downloads: 30   Since Mar. 13, 2018 Views: 441   Since Mar. 13, 2018
Authors
[1]
Mika Koizumi, Research Institute for Science and Engineering, Waseda University, Tokyo, Japan.
[2]
Hiromi Kano, Oak-Hill Georgic Patch-Work Laboratory, Chiba, Japan.
Abstract
Pea seedlings (Pisum sativum L.) were cultured using moist tubes with wet gauze cloth. Manganese ion uptake and transport in the root were observed using a dedicated micro-magnetic resonance imaging (MRI) device equipped with a 1.0-Tesla (T) permanent magnet, and 30-mm and 20-mm solenoid coil detectors. Signal enhancement with absorbed paramagnetic manganese ion on T1-weighted images was tracked using time-lapse imaging. Differential maximum intensity projection (MIP) and slice images were examined to discern movement of manganese between individual root tissues. Long-distance transport of the ion along roots and radial movement of the ion from outside to the stele through the cortex of roots were visualized. Manganese ion was transported through the stele when the ion was applied from a lower level than the observation position. Manganese was taken up rapidly near the root tip, and the velocity of uptake became slow depending on the distance from the root tip when measurements were carried out for immersed roots in manganese solution. The root apex, where enhancement was weak, seemed to have a different property than other parts of the root. Ions first filled the cortex and then moved to the stele in the transverse sections. Enhanced signals remained in the stele for a while after the enhancement disappeared from the cortex.
Keywords
Ion Uptake and Transport, Magnetic Resonance Imaging (MRI), Manganese Solution, Signal Enhancement with Paramagnetic Ion, Pea Plant (Pisum sativum L.), Root Positions
Reference
[1]
Borisjuk, L., Rolletschek, H. and Neuberger, T. Surveying the plant’s world by magnetic resonance imaging. Plant J 2012; 70: 129-146.
[2]
Van As, H. and van Duynhoven, J. MRI of plants and foods. J Magn Reson 2013; 229: 25-34.
[3]
Brown, J. M., Kramer, P. J., Cofer, G. P. and Johnson, G. A. Use of nuclear magnetic resonance microscopy for noninvasive observations of root-soil water relations. Theor Appl Climatol 1990; 42: 229-236.
[4]
MacFall, J. S., Johnson, G. A. and Kramer, P. J. Observation of a water-depletion region surrounding loblolly pine roots by magnetic resonance imaging. Proc Natl Acad Sci, USA 1990; 87: 1203-1207.
[5]
MacFall, J. S., Johnson, G. A. and Kramer, P. J. Comparative water uptake by roots of different ages in seedlings of loblolly pine (Pinus taeda L.). New Phytol 1991; 119: 551-560.
[6]
Terskikh, V. V., Feurtado, J. A., Ren, C., Abrams, S. R. and Kermode, A. R. Water uptake and oil distribution during imbibition of seeds of western white pine (Pinus monticola Dougl. ex D. Don) monitored in vivo using magnetic resonance imaging. Planta 2005; 221: 17-27.
[7]
Pohlmeier, A., Oros-Peusquens, A., Javaux, M., Menzel, M. I., Vanderborght, J., Kaffanke, J., Romanzetti, S., Lindenmair, J., Vereecken, H. and Shah, N. J. Changes in soil water content resulting from Ricinus root uptake monitored by magnetic resonance imaging. Vadose Zone J 2008; 7: 1010-1017.
[8]
Rascher, U., Blossfeld, S., Fiorani, F., Jahnke, S., Jansen, M., Kuhn AJ., Matsubara, S., Märtin, LLA., Merchant, A., Metzner, R., Müller-Linow, M., Nagel, KA., Pieruschka, R., Pinto, F., Schreiber, CM., Temperton, VM., Thorpe, MR., van Dusschoten, D., van Volkenburgh, E., Windt, CW. and Schurr, U. Non-invasive approaches for phenotyping of enhanced performance traits in bean. Funct Plant Biol 2011; 38: 968-983.
[9]
Hillnhütter, C., Sikora, R. A., Oerke, E.-C. and van Dusschoten, D. Nuclear magnetic resonance: a tool for imaging belowground damage caused by Heterodera schachtii and Rhizoctonia solani on sugar beet. J Exp Bot 2012; 63: 319-327.
[10]
Farrar, T. C. and Becker, E. D. Pulse and Fourier transform NMR: Introduction to theory and methods. Academic Press, New York and London, 1971.
[11]
Bottomley, P. A., Rogers, H. H. and Foster, T. H. NMR imaging shows water distribution and transport in plant root systems in situ. Proc Natl Acad Sci, USA 1986; 83: 87-89.
[12]
Connelly, A., Lohman, J. A. B., Loughman, B. C., Quiquampoix, H. and Ratcliffe, R. G. High resolution imaging of plant tissues by NMR. J Exp Bot 1987; 38: 1713-1723.
[13]
Koizumi, M., Ishida, N. and Kano, H. Imaging Mn2+ uptake of a maize shoot by an NMR microscope. Bot Mag Tokyo 1992; 105: 667-671.
[14]
Bourgeois, D and Decorps, M. Quantitative imaging of slow coherent motion by stimulated echoes with suppression of stationary water signal. J Magn Reson 1991; 94: 20-33.
[15]
Kuchenbrod, E., Kahler, E., Thürmer, F., Deichmann, R., Zimmermann, U. and Haase, A. Functional magnetic resonance imaging in intact plants – quantitative observation of flow in plant vessels. Magn Reson Imaging 1998; 16: 331-338.
[16]
Koizumi, M. and Kano, H. Tracking manganese ion uptake and transport by the root system of broad bean plants by micro-magnetic resonance imaging. Amer J Biol Life Sci 2015; 3: 102-107.
[17]
Street, H. E. and Cockburn, W. Plant metabolism, 2nd ed. 1972; Oxford Pergamon Press.
[18]
Windt, C. W., Vergeldt, F. J., De Jager, P. A. and Van As, H. MRI of long-distance water transport: a comparison of the phloem and xylem flow characteristics and dynamics in poplar, castor bean, tomato and tobacco. Plant Cell Environ 2006; 29: 1715-1729.
[19]
Kano, H., Koizumi, M. Seasonal changes in the dynamic state of water for excised cherry branches (Prunus lannesiana) observed using dedicated micro-magnetic resonance imaging. Plant 2014; 2: 60-67. doi: 10.11648/j.plant.20140206.11.
Open Science Scholarly Journals
Open Science is a peer-reviewed platform, the journals of which cover a wide range of academic disciplines and serve the world's research and scholarly communities. Upon acceptance, Open Science Journals will be immediately and permanently free for everyone to read and download.
CONTACT US
Office Address:
228 Park Ave., S#45956, New York, NY 10003
Phone: +(001)(347)535 0661
E-mail:
LET'S GET IN TOUCH
Name
E-mail
Subject
Message
SEND MASSAGE
Copyright © 2013-2017, Open Science Publishers - All Rights Reserved