Uptake and Transport of Manganese Ion in Relation to the Positions of a Root for Pea Plant Observed by Micro-magnetic Resonance Imaging

Mika Koizumi; Hiromi Kano

doi:7040346

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Uptake and Transport of Manganese Ion in Relation to the Positions of a Root for Pea Plant Observed by Micro-magnetic Resonance Imaging

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Volume 6, 2018
Issue 1 (February)

Pages: 8-13 | Vol. 6, No. 1, February 2018 | Follow on

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Authors

[1]

Mika Koizumi, Research Institute for Science and Engineering, Waseda University, Tokyo, Japan.

[2]

Hiromi Kano, Oak-Hill Georgic Patch-Work Laboratory, Chiba, Japan.

Abstract

Pea seedlings (Pisum sativum L.) were cultured using moist tubes with wet gauze cloth. Manganese ion uptake and transport in the root were observed using a dedicated micro-magnetic resonance imaging (MRI) device equipped with a 1.0-Tesla (T) permanent magnet, and 30-mm and 20-mm solenoid coil detectors. Signal enhancement with absorbed paramagnetic manganese ion on T₁-weighted images was tracked using time-lapse imaging. Differential maximum intensity projection (MIP) and slice images were examined to discern movement of manganese between individual root tissues. Long-distance transport of the ion along roots and radial movement of the ion from outside to the stele through the cortex of roots were visualized. Manganese ion was transported through the stele when the ion was applied from a lower level than the observation position. Manganese was taken up rapidly near the root tip, and the velocity of uptake became slow depending on the distance from the root tip when measurements were carried out for immersed roots in manganese solution. The root apex, where enhancement was weak, seemed to have a different property than other parts of the root. Ions first filled the cortex and then moved to the stele in the transverse sections. Enhanced signals remained in the stele for a while after the enhancement disappeared from the cortex.

Keywords

Ion Uptake and Transport, Magnetic Resonance Imaging (MRI), Manganese Solution, Signal Enhancement with Paramagnetic Ion, Pea Plant (Pisum sativum L.), Root Positions

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