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The Effect of the Combination of Cetuximab (Erbitux®) and Sodium Butyrate on Mucous Secreting Cells during Rat Colon Carcinogenesis
Current Issue
Volume 6, 2018
Issue 2 (June)
Pages: 18-30   |   Vol. 6, No. 2, June 2018   |   Follow on         
Paper in PDF Downloads: 21   Since Jul. 23, 2018 Views: 460   Since Jul. 23, 2018
Authors
[1]
Farida Elmoursi Elbassiouni, Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt.
[2]
Elsayed Ibrahim Salim, Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt.
[3]
Doaa Hussein Zineldeen, Department of Biochemistry, Faculty of Medicine, Tanta University, Tanta, Egypt.
Abstract
Colon carcinogenesis is a multistep process which originates from a series of histopathological and molecular alterations. To study the effect of the combination of Cetuximab (Erbitux) and Sodium butyrate and the role of mucous secreting cells during short –term rat colon carcinogenesis, we used male Wistar rats divided into 5 groups. Group 1: Normal control rats, group 2: 1,2-dimethylhydrazine (DMH)- injected rats, group 3: Rats injected with DMH then received Cetuximab at a dose of 10 mg/kg body weight for two weeks in the 5th and 8th week of start, group 4: Rats injected with DMH then treated with Na-butyrate, i.p, 200mg/kg body weight from the 5th week till the end, and group 5: Rats injected with DMH then treated with both Cetuximab and Na-butyrate at the same doses and protocols as in groups 3 and 4. The rats from groups 3, 4 had significantly lower numbers of colonic cancer biomarkers; namely aberrant crypt foci (ACF) and mucin depleted foci (MDF) as compared with DMH group 2. Interestingly, the Cetuximab and Na-Butyrate-treated group 5 had significantly the lowest numbers of ACF and MDF as compared with DMH control, as well as retained the goblet cell numbers in the colonic crypts almost to control levels.
Keywords
Colon Carcinogenesis, Cetuximab, Erbitux, Sodium Butyrate, Aberrant Crypt Foci, Mucin Depleted Foci
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