Study of DNA Fragmentation in Gametes of Infertile Male by Using TUNEL Technique
[1]
Ouafia Liamani, Department of Biology, Faculties of Nature and Life Sciences, Djillali Liabes University, Sidi Bel Abbes, Algeria.
[2]
Soraya Moulessehoul, Department of Biology, Faculties of Nature and Life Sciences, Djillali Liabes University, Sidi Bel Abbes, Algeria.
[3]
Ismat Benzeguir, Laboratory of Analysis of Medical Biology, Sidi Bel Abbes, Algeria.
Male factors leading to infertility account for at least half of all cases of infertility worldwide. In this study, we used Terminal desoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay to evaluate DNA fragmentation in sperm cells. The study group included 12 males with infertility and oligoasthenoteratozoospermia (OATs). The mean DNA fragmentation index was significantly higher in patients compared to the control group. In conclusion the TUNEL may be considered as an additional assay for the evaluation of spermatozoa beside standard analysis, thus playing an important role during proper diagnosis and treatment of infertility. As in these days, for patients with OAT, intracytoplasmic sperm injection (ICSI) is frequently used.
Oligoasthenoteratozoospermia, DNA Fragmentation, TUNEL
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