Regeneration of Potato Plantlets Through Shoot Tip Culture Comparison Between GA3 and BAP
The research was conducted to investigate on the regeneration of potato plantlets through shoot tip culture, were carried out in the Virology Laboratory of Plant Pathology Section. Agriculture Research Institute, Tandojam during 2012. The regeneration of potato plantlets was compared between media based on various BAP and GA3 concentrations. The results revealed significant (P<0.05) effect of Murshigue & Skoog (M S) media and their concentrations on root/shoot number, root/shoot length, leaves plant-1 and callus formation. It was also observed that GA3 based media at 2.00mg liter-1 concentration to produce roots plant-1 34.65, shoots plant-1 11.48, cm root length 4.13, cm shoot length 5.56, leaves plant-1 6.37 and percent callus formation 83.12; while the values for these characters under rest of the GA3 concentrations (2.50, 1.50 and 1.00 mg liter-1 were significantly lower than this optimum GA3 concentration. In BAP based media, 4.00 mg liter-1 concentration showed better results with 24.38 roots plant-1, 7.07 shoots plant-1, 3.44 cm root length, 4.29 cm shoot length, 5.29 leaves plant-1 and 66.63 percent callus formation; and values for these traits for other BAP concentrations were significantly lower. On an overall average, the BAP and GA3 based media resulted in 21.20 and 28.70 roots plant-1, 5.60 and 8.72 shoots plant-1, 2.58 and 2.92 cm root length, 2.93 and 3.86 cm shoot length, 3.94 and 4.83 leaves plant-1 and 54.87 and 67.60 percent callus formation. In conclusion, results clearly indicate that GA3 was more effective to produce higher values for all the traits examined for regeneration of plantlets through shoot tip culture.
Regeneration, Potato Plantlets, Nitrogen, Shoot Tip Culture, BAP, and GA3
Real Academia Española Retrieved 16 July (2010). Dictionaries Usual" (in Spanish). Buscon. raise. Retrieved 16 July 2010.
Miller, N 29 January (2008). Using DNA, scientists hunt for the roots of the modern potato". American Association for the Advancement of Science. Retrieved 10 September 2008.
Bodoni, A, and J. S. Chauhan (2009). Effect of Growth Regulators on Meristem-tip Development and in-vitro Multiplication of Potato Cultivar ‘Kufri Himalini’, Nature and Sci., 7(9): 31-34.
Hawkes, J. G. (1978). Biosystematics of the potato. In: “The potato crop” (P. M. Harris, ed.) Halstead Press, New York. PP. 15-69.
John Roach, 10 June (2002). Saving the Potato in its Andean Birthplace". National Geographic. Retrieved 11 September 2009.
Bodoni, A. and J. S. Chauhan. (2010). In-Vitro Sterilization Protocol for Micro propagation of Solanum tuberous cv. Kufri Himalini’ Aca. Arena, 2 (4): 24-27.
Adkins et al., of up to 99% CIP (2015). Sweet potato cultivar degeneration rate under high and low potato virus disease pressure zones in Uganda. Canadian Journal of Plant Pathology, 37: 1, 136-147.
Gopal, J. and N. S. Chauhan. (2010). Slow growth in-vitro conservation of potato germplasm at low temperature. Potato Res., 53: 141-149.
Hossain, M. J. (1994). In-vitro propagation of potato (Solanum tuberosum L.). J. Plant Tissue Cult., 1: 33-37.
Ehsanpour and Jones, (2000). Evaluation of direct shoot regeneration from stem explants of potato (Solanum tuberosum L.) cv. Delaware by Thidiazuron (TDZ). J. Sci. Tech. Agric., 3: 47-54.
Rind, G. R. (2007). The effects of sugar and different growth regulators on suberization of potato (Solanum tuberosum L.) cuttings. M. Sc. Thesis submitted to Sindh Agriculture University Tandojam.
Otero et al. (2012). Low-Cost Tissue Culture Technology in the Regeneration of Sweet Potato (Ipomoea batatas (L) Lam). Res. J. of Biol., 2 (2): 51-58.
Sparsed, B. J., and A. Gubba. (2012). Development of an efficient plant regeneration protocol for sweet potato (Ipomoea batatas L.) cv. Blesbok. African J. of Biotec., 11(84): 14982-14987.
Ashanti, M., M. Kharrazi, A. Sharifi and M. Mehrvar. (2012). Carnation etched ring virus elimination through shoot tip culture. J. Biol. Environ. Sci., 6 (17): 175-180.
Danci, O. Daniela, P. Cerasela, F. Berbentea and I. David. (2012). Production of virus free potato plantlets. J. of Hort., Forestry and Biotic., 16(1): 232-238.
Ullah, I., M. Jadoon, A. Rehman, T. Zeb and K. Khan. (2012). Effect of Different GA3 Concentration on in-vitro Propagation of Potato Variety Desiree, Asian J. of Agric. Sci., 4 (2): 108-109.
Padma, M. M., and G. A. Ravishankar. (2013). In-vitro propagation and genetic fidelity study of a plant regenerated from inverted hypocotyl explants of eggplant (Solanum melongena L.) cv. Arka Shirish. Biotech, 3(1): 45–52.
Shibli, R. A., A. M. Abu-Ein, and M. M. Ajlouni. (2001). In-vitro and in-vivo multiplication of virus-free 'Spunta' potato, 33(1): 35-41
Ghafoor, A., G. B. Shah and K. Waseem. (2003). The in-vitro response of potato (Solanum tuberosum L.) to various growth regulators. Biotic., 2 (3): 191-197.
Muller, S. A. and L. Lipschutz. (1984). Potato. In: Amaretto, P. V., D. A. Evans, W. R. Sharp and Y. Yamada, (Eds.), Handbook of Plant Cell Culture, Collier Mcmillan Publishers, London, 3: 295.
Roast and Bokelmann (1976). Vegetative propagation of Solanum tuberosum L. in-vitro. Potato Res., 19: 173-178.