Abiotic Stress and Its Impact on Protein Concentration or Polymorphism of Gloriosa superba Plant
[1]
Dharmendra Singh, Department of Botany, Govt. M. V. M., Barkatullah University, Bhopal, India.
[2]
Manish Mishra, Department of Ecosystem Management, IIFM, Bhopal, India.
[3]
Anirudha Singh Yadav, Department of Botany, Govt. M. V. M., Barkatullah University, Bhopal, India.
Biotic and abiotic stresses exert a considerable influence on the production of several secondary metabolites in plants. Temperature, pH and light are one of the important abiotic stress that affected survival, growth, reproduction and geographic distribution of crop plants. Biochemical characterization of species and their genetic association and polymorphism within the related species based on morphological data is becoming difficult because these morphological traits are highly influenced by the environment. Proteins play significant biological function in human as well in the plants. In this study, G. superba cultured in MS medium under abiotic stress. To study the effect of stresses on protein concentration, protein extracted and quantified by Lowry method. Total levels of protein were found to be varied in plants grow under different temperature. Maximum 11.308 µg/100mg was observed in plant cultured at 25°C temperature and minimum amount was 4.791 µg/100mg at 35°C temperature. pH and photoperiod does not exist more difference in protein concentrations. To investigate the molecular weight of proteins, a standard protein marker was used. Electrophoresis of proteins has been successfully used for the characterization of different taxonomic, evolutionary and genetic relationship studies. In the present study, the electrophoratic banding profile of total soluble proteins of G. superba plant cultured under different abiotic stress exhibited presence versus absence type of polymorphism. The present investigation of SDS denatured proteins showed differences in a number of bands, bandwidth and intensity and exhibited genetic diversity between all variants. This study is very important for researcher, who is working in the field of climate change. By this research we can conserve endangered plant species in challenging environment and can enhance important plant chemical constitute under stress conditions.
Abiotic Stress, Protein Concentration, Protein Polymorphism, Micropropagation, Gloriosa superba
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