Phytochemical Screening of Various Species of Cola Nut Extracts for Antifungal Activity against Phytopathogenic Fungi
[1]
A. I. Kanoma , Department of Biology, Zamfara State College of Education Maru, Maru, Zamfara State, Nigeria.
[2]
I. Muhammad , Department of Chemistry, Zamfara State College of Education Maru, Maru, Zamfara State, Nigeria.
[3]
I. Ibrahim D. , Department of Chemistry, Zamfara State College of Education Maru, Maru, Zamfara State, Nigeria.
[4]
K. Shehu , Department of Biological Sciences, Usmanu Dan Fodiyo University, Sokoto, Nigeria.
[5]
H. M. Maishanu , Department of Biological Sciences, Usmanu Dan Fodiyo University, Sokoto, Nigeria.
[6]
A. D. Isah , Department of Biological Sciences, Usmanu Dan Fodiyo University, Sokoto, Nigeria.
The antifungal activities of aqueous and methanol nut extracts of C. nitida and C. acuminata against phytopathogenic fungi namely; Aspergillus niger, Aspergillus fumigatus, Rhizopus oryzae, and Mucor racemosus were investigated using agar incorporation method. A significant difference (p<0.05) in extracts concentration activities was observed at varying concentrations (mg/ml) of 40, 60, 80 and 100. The highest percentage inhibition and extract sensitivity was recorded 82.96 in Rhizopus oryzae at 100mg/ml of aqueous extract of C. nitida and 81.2 for aqueous extract of C. acuminata at 100mg/ml respectively. In methanol extract Rhizopus oryzae shows a percentage growth inhibition of 72.76 in C. nitida and 71.85 in C. acuminata at 100ml/mg respectively. For Mucor racemosus, the percentage growth inhibition was 73.70 and 72.40 for the aqueous extracts of C. nitida and C. acuminata at 100mg/ml, while the methanol extracts was 66.66 and 66.29 at 100mg/ml for C. nitida and C. acuminata respectively. Aspergillus fumigatus shows 29.25 and 31.60 percentage inhibition for the aqueous extracts of C. nitida and C. acuminata at 100mg/ml, while in 100mg/ml of methanol extracts, the percentage inhibition was 40.88 and 38.88 for C. nitida and C. acuminata respectively, and there is no inhibition at 60 and 40mg/ml of the aqueous and methanol extracts of C. nitida and C. acuminata respectively. For Aspergillus niger at 100mg/ml of aqueous extact, the percentage growth inhibition was 69.62 and 65.52 for C. nitida and C. acuminata, while in 100mg/ml of methanol extracts, the percentage growth inhibition was 65.18 and 65.10 for C. nitida and C. acuminata respectively, and there is no inhibition at 60 and 40mg/ml of the aqueous and methanol extracts of C. nitida and C. acuminata respectively. In all the extracts, the percentage growth inhibition increases with increase in concentration of extracts in all the test organisms. However, from the two extracts used, they have shown a closely related percentage growth inhibition against all the isolate organism. Finally, the finding from this study reveals a rich source of bioactive compounds essentials in diseases treatment, and suggest the use of the extracts in herbal curl remedies.
Cola Nitida, Cola Acuminata, Anitfungal Activy
[1]
Ali-Shtayeh, M. S., Abu, S. I. and Ghdeib, G. (1999). Antimycotic activity of twenty-two plants used in Folkloric medicine in the Palestinian area for the treatment of skin diseases suggestion of dermatophyte infection. Mycoses, 42: 665-672.
[2]
Amusa, N. A. And Odumbaku, O. A. (2007). Biological Control of Bacterial diseases of plants in Nigeria: Problem and Prospect. Research Journal of Agriculture and Biological Sciences. 316:979-982.
[3]
Bejamin, L. T., Rogers, A. M., Rosenbaum, A. (1999). “Coca cola” Caffeine and Mental Deficiency Harry Holingrowth and the chattanoogatrial of 1991” J histol Behavioural Sci. 27(1):42-45.
[4]
Duke, J.A. (2001). Handbook of Nuts. Boca Raton, Fe: CRC Press; p.343.
[5]
Evans, W. C. (1999). Trease and Evans Pharmacognosy 14th Edition W. B. Saunders Company Limited, New York pp1-340.
[6]
Farambi, E. O. (2003). African Indigenous Plant with Chemotherapeutic Potential and Biotechnological approach to the Production of Bioactive prophylactic agents. African J. Biotech 2:662-671.
[7]
Jeyeola, O.C. (2001). Preliminary Studies on the use of Kolanut (Colanitida) for soft drink production J. Food Technol Afr. 6(1):25-26.
[8]
Kim, M.K.., Choi, G. J. And Lee, H.S. (2003). Rhizomederived Curcumin Against phytopathogenic fungi in Green House. Journal of Agricultural and Food Chemistry 51: 261-264.
[9]
Kuome, C., Sacande, M. (2006). Cola nitida (vent) scholt and end1 seed leaflet:111.
[10]
Ndukwe, K.C., Okeke, I. N. Lamkanra, A, Adesina,S. K. Aboderin, O.J. (2005). Antibacterial activities of aqueous extracts of selected chewing sticks. J. Contemp. Dent Practical 3: 86-94.
[11]
Newall, C. A., Anderson, L. A., Phillipson, J. D. (1996). Herbal Medicine a Guide for Health Professional London. The Pharmaceutical Press; p.84.
[12]
Ojanuga, A.G. (2006). Agreocological zones of Nigeria Manual. National programme for Food Security FAO. Abuja Nigeria. 124Pp.
[13]
Tiew, P., Loset, J.R., Kokpol, U., Chavasiri, W. Hostettman, K. (2003). Antifungal, Antioxidant and Lavicidal Activities of Composed Isolated from the Heart Wood of Mansonia gagei; Phytother Res (2); 190-193 [Plumed].
[14]
Wink, M. (1993). Production and Application of Phytochemicals from Agricultural Perspective in: T.A. Van Beek and H. Breteler (eds), Europe: Photochemistry and Agriculture, Clarendor press, oxford pp 171-213.
