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Maziar Bahreini, Department of Biochemistry, Faculty of Biological Sciences, Tehran North Branch, Islamic Azad University, Tehran, Iran.
[2]
Mansour Omidi, Department of Biotechnology, Faculty of Agronomy and Plant Breeding, Tehran University, Karaj, Iran.
[3]
Fereydoon Bondarian, Department of Biotechnology and Plant Breeding, Faculty of Agriculture Science and Research Branch, Islamic Azad University, Tehran, Iran.
[4]
Majid Gholibaygian, Razi Laboratory Complex, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Fennel as annual or biennial aroma plants which widely grown in Mediterranean and some tropical regions and it uses for herbal drug preparations. In this work n-Hexane extract constituents of in vitro plantlets were identified in Nano elicited (Tio2 and Sio2 particles) and normal conditions using Thin Layer Chromatography (TLC) by Toluene: ethyl acetate (93:7) as solvent system for Mobile phase and Gas Chromatography-Mass Spectroscopy (GC-MS) by Retention indices calculation using Homologous series of n-alkanes C8 –C19 , the major components of normal plant were t-anethole, Fenchone and Limonene and Decane were the most frequent component. Rf =0.92 was calculated as semi quantitative parameter for t-anethole detection, some identified constitutes of Tio2 elicited plant extract were Dodecane, Phytol and Phenol 2,4 bis (1,1 dimethyl ethyl), and the most frequent compound was Octane. Also benzoic acid, Jasmonol and Hexadecanoic acid were detected as Sio2 elicited plants components and major compound was Pyrrolidione.
GC-MS, Aroma Plants, n-Hexane, Retention Index
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