Seed Culture of Aromatic Rice Varieties Under Salt Stress
Dehusked seeds of five aromatic rice varieties were placed on MS medium supplemented with NaCl and Na2SO4 salts at four different levels (0%, 0.2%, 0.4% and 0.6%) to observe the callus induction and plant regeneration responses. For callus induction MS medium was supplemented with 2 mg/L 2, 4 D while for plant regeneration MS medium was supplemented with 1 mg/L NAA and BAP. All the genotypes presented significant variation in callus induction and plant regeneration responses. Callus induction and plant regeneration percentage decreased with the increase of salt doses. Among the tested varieties, in most of the cases, Kalijira performed the best both for callus induction and plant regeneration at different levels of salts (0 - 0.6%). It was concluded that salt tolerant aromatic rice varieties can be evolved applying salt stress in in vitro condition.
Callus Induction, Plant Regeneration, Salinity, Somaclonal Variation
Adil M, Hasan M, Kamal M M, Zakaria M and Mian M A K. 2009. In vitro plant regeneration of aromatic rice under salt stress. Intl. J. BioRes. 6 (6): 48-52.
Aditya T L and Baker D A. 2005. The effect of continuous NaCl stress on the restoration of high callus induction frequency, regeneration potential and in vitro growth of somaclones of four Bangladeshi indica rice genotypes. Oryza. 42: 103-108.
Anbazhagan M, Rajendran R, Kalpana M, Natarajan V and Panneerselvam R. 2009. Agrobacterium mediated Transformation of rice, var. Pusa Basmati - 1. J. Ecol. Biotechnol. 1: 7-11.
Blumwald E A and Grover A. 2006. Salt tolerance in Plant Biotechnology: Current and Future uses of Genetically Modified Crops. John Wiley and Sons Ltd., UK pp. 206-224.
Dang M T and Lang N T. 2003. In vitro selection for salt tolerance in rice. Omonrice. 11: 68-73.
Hasan M and Sarker R H. 2013. In vitro selection for NaCl salt tolerance in aromatic rice genotypes. Indian J. Agril. Sc. 83(11): 109-114.
Kumari R, Sharma V K and Kumar H. 2015. Seed culture of rice cultivars under salt stress. Int. J. Pure App. Biosci. 3 (1): 191-202.
Murashige T and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473 - 497.
Ping W, Gang W and Jing J. 2006. NaCl tolerance of different genotypes in rice under tissue culture. J. Jilin Agril. Univ. 28: 601-605.
Sarker N S, Hasan M, Rahman M M, Mian M A K and Adil M. 2010. In vitro selection for salt tolerance in aromatic rice genotypes. J. Agric. & Env. Sci. 2: 25-28.
Shavindra J and Amitabh M. 2005. Advances in rice biotechnology in the new millennium. Plant Biotechnol. J. 3: 275-307.
Tariq M, Ali G, Hadi F, Ahmad S, Ali N and Shah A A. 2008. Callus induction and in vitro plant regeneration of rice (Oryza Sativa L.) under various conditions. Pakistan J. Biol. Sc. 11: 255-259.
Zahid M N, Hasan M, Adil M, Hossain M M and Mian M A K. 2014. In vitro screening for salt tolerance in aromatic rice genotypes. Open Science Journal of Bioscience and Bioengineering. 1 (2): 28-32.