Combined Effect of Explant, Inoculation Time and Co-cultivation Period on Agrobacterium - Mediated Genetic Transformation in Cucumber (Var. Shital)
Combined effect of explant, inoculation time and co-cultivation periodon Agrobacterium-mediated genetic transformation in cucumber was observed. Transformation ability was examined by histochemical assay of GUS reporter gene in survived calli. Conspicuous GUS positive (blue colour) region were detected in callus tissue. There were 3 factors in this investigation. Factor A consisted of three types of explants viz. leaf, nodal and internodal callus, factor B consisted of two durations of inoculation time viz. 3 and 5 min and factor C consisted of two co-cultivation periods viz. 24 and 48 hours. The highest number (4.04) and percentage (67.38%) of GUS +vecalliwas obtained from leaf explants when they were immersed in Agrobacterium suspension for 5 min and kept into co-cultivation media for 48 hrs.The lowest number(0.95) and percentage (15.89%) of GUS +ve calluswere observed in internodal explants when they were inoculated in Agrobacterium for 3 min and then co-cultivated for 24 hrs.
Cucumber, Genetic Transformation, Abiotic Stress
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