Association of Typing HLA with Toxoplasmosis in Iraqi Women Patients
[1]
Yassir Dakheel Kremsh Alasadiy, Department of Biology, College of Science, University of Al - Muthanna, Ministry of Higher Education & Scientific Research, AL-Samawa city (Southern Iraq), Iraq.
[2]
Hawraa Hamza Abbas, Department of Biology, College of Science, University of Al - Muthanna, Ministry of Higher Education & Scientific Research, AL-Samawa city (Southern Iraq), Iraq.
Toxoplasma gondii is an obligate intracellular protozoan responsible for common parasitic infections throughout the world. It can invade and multiply in the nucleated cells of virtually all worm blooded animals including humans, domestic mammals, and birds, with members of the cat family being the only known hosts for the sexual stages of T. gondii infection, it is a world - wide disease results in serious health and financial loss. Iraq considered as an endemic region for this disease. One hundred fifty women were included in this study with a history of single or repeated abortion that was referred by physicians to detect anti-Toxoplasma antibodies. The study included one hundred women with spontaneous abortion and 25 women with normal pregnancy and 25 healthy women were used as a control. Blood samples were collected for extraction of DNA and amplification by Real-Time PCR technique and HLA typing of injured and healthy control groups, our work studying the associations between Human Leukocyte Antigen (HLA) (i.e. human major histocompatibility complex (MHC) genes and susceptibility to infections with toxoplasmosis have been described. The present study showed the percentage of the HLA-DQA1 and HLA-DQB1 alleles between the groups, there were decreased percentage of the HLA-DQA1*0302 and HLA-DQB1 *0501 alleles in the patient group compared to the control group (37.5% v 62.5%), (16.7% vs 70.8%) respectively, but the precentage of the HLA-DRB1*1302 explained increased in patients with compared to the with control (66.7 % vs 37.5%) respectively.
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