Comparative In-vitro Activity of Cefuroxime and Augmentin on Staphylococcus aureus Isolates From Nostrils of Healthy Undergraduate Students of Ambrose Alli University, Ekpoma
[1]
Orhue O. P., Department of Microbiology, Faculty of Natural Sciences, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
[2]
Oikhala O. G., Department of Microbiology, Faculty of Natural Sciences, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
[3]
Agbolahor D. E., Department of Microbiology, Faculty of Natural Sciences, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
[4]
Ekundayo A. O., Department of Microbiology, Faculty of Natural Sciences, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
[5]
Oviasojie F. E., Department of Microbiology, Faculty of Natural Sciences, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
The activities of antibiotics are known to differ in pharmacokinetic, pharmacodynamic as well as in the mode and mechanism of action and this create some difficulties in selecting the preferred antibiotic for different bacterial infections. The situation is further made worsen with the development of antibiotic resistance by many bacterial agents and organisms. Based on this background, this study was undertaken to compare the in-vitro activities of Cefuroxime and Augmentin antibiotics indicated by their minimum inhibitory concentration (MIC) on nasal Staphylococcus aureus (S. aureus) isolates. The study was conducted in the Microbiology Laboratory of the Department of Microbiology, Ambrose Alli University, Ekpoma in Edo State, Nigeria. The S. aureus isolates used were isolated from the nostril of students in the Department of Microbiology following due ethical processes. Following standard laboratory procedures, the minimum inhibitory concentration (MIC) of Cefuroxime and Augmentin were determined in 12 confirmed S. aureus isolates and the values recorded and compared using the paired sample ‘t test’ of SPSS version 20 at 95% confidence interval. The results showed that Cefuroxime (0.38±0.32 µg/ml) antibiotic has the lowest mean MIC compared to Augmentin (5.16±5.69 µg/ml). While the MIC ranges from 0.02µg/ml to 0.64µg/ml for Cefuroxime antibiotic, it was between 0.05µg/ml to 12.50µg/ml for Augmentin antibiotic. Although both drugs showed antimicrobial potentials against nasal S. aureus, based on the findings of this study, Cefuroxime is a better antibiotic for treating respiratory S. aureus infection compared to Augmentin.
Comparative Antibiotics Activity, Cefuroxime, Augmentin, Staphylococcus aureus
[1]
Adeleke O E, Ogunniyi O A and Olarinde J D. Susceptibility of some Gram-positive and Gram-negative bacteria against some brands of amoxicillin-clavulanic acid. N Y Sci J 2014;7(6):80-86.
[2]
Adesida, S.A., Abioyi, O.A., Banero, B.S. ., Brai, B.T.C., Smith, S.I., Amisu, K.O., Ehichioya, D.U., Ogunsola, E.T. and Coker, A.O. (2007). Associated risk factors and pulse-field gel electrophoresis of nasal isolated of Staphylococcus aureus from medical students in a tertiary hospital in Lagos, Nigeria. Brazilian J Infect. Dis., 11(1):
[3]
Agbonlahor, D.E and Adegbola, R.A. Mechanism of bacterial resistance to antibiotics. In: Uzoma, K.C., Nwobu, R; Adedeji, S.O. eds Medical Bacteriology 2nd ed. Commercial Press. Benin City. 1996, Pp 89-91.
[4]
Andrews, J.M. (2001). “Determination of minimum, inhibitory concentrations” J. of Antimicro. Chemo. 48(1):5-16.
[5]
Baliga, S., Bansil, R., Suchitra, V., Bharati, B., Vidyaniketan, K. and Shenoy, S. (2007). Nasal carriage of MRSA in medical students. J. Hosp. Infect., 91-92.
[6]
British National formulary (54 ed.) September, 2001.
[7]
British National Formulary. Infection. British Medical Association and Royal Pharmaceutical Society of Great Britain London: UK. 1999; pp 246.
[8]
Brunett, A.F., Stockill and Quinn, G.A. (1996). “Suppression of innate immunity by a nasal carriage strain of staphylococcus aureus increases its colonization of nasal epithelium”. Immuno 122(1): 80-9.
[9]
Calderon, C.B. and Sabundayo, B.P. (2007). “Antimicrobial classification”: Dr. for. Bug.10(2): 99-123.
[10]
Cruickshank, R., Duguid, J.P., Marmiun, J.P and Swam, R.H. (1975), Staphylococcus aureus, Medical Micro biology -13th edition. Livingston Publishers Pp 236-245.
[11]
Gabbum, J.H. (1990). Theories of Drug Antagonism.Pharm. 9(3): 211-218.
[12]
Hiramatsu, K., Hanak, H., Ino, T. and Yenover, F. (1997). “Methicillin – resistant staphylococcus aureus clinical strain with reduced vancomycin susceptibility”. Antimicrob. Chemo. 40(1):135-6.
[13]
Karchmer, A.W. (2000). Cephalosporins, Principles and practices of infectious diseases.Clin. Infect. Dis. pp 274-291.
[14]
Kluytmans, J. (1997). “Nasal carriage of epidemiology, underlying mechanisms and associated risks”. Clin. Microbiol. Rev. 10(3):805-20.
[15]
Kucers, A., and Benelt, N. (2004).The use of Antibiotics.A comprehensive Review with clinical emphasis Pp. 201-208.
[16]
Lamikanra, B. D. Paul, O. B. Akinwole and M. O. Paul (2006) Nasal carriage of Staphylococcus aureus in a population of healthy Nigerian students., J Med Microbiol 55 , 317-324.
[17]
Lindblad.W.J. (2008).“Considerations for determining if a natural product is an effective wound-hesling agent.Int. J. of lower. Ext. Wound. 7(2):75-81.
[18]
Momoh, A.R.M., Orhue, P.O., Idonije, O.B., Oaikhena A.G., Nwoke E.O. and Momoh, A.A. (2011). The antibiogram types of Escherichia Coli isolated from suspected urinary tract infection samples. J. Microbiol. Biotech. Res., 1 (3): 57-65.
[19]
Orhue, P.O. and Momoh A.R.M. (2012). The antibiogram types of Staphylococcus aureus isolated from nasal carriers from irrua Specialist teaching hospital, Edo state, Nigeria. E3 Journal of Biotechnology and Pharmaceutical Research Vol. 3(4), pp. 83-87.
[20]
Podolsky, M. and Lawerence, J. (1998). “Cures out of chaos”: How unexpected discoveries led to break through in medicine and health Harwood Academic publishers. Pp 54-78.
[21]
Santhosh,DV., Shobha,KL.,Bairy,I.,Rao,G.,Anand,KM and D’ Souza,J: Nasal screening and survey of pre-clinical medical students from Malaysia for nasal carriage of coagulase positive MRSA and rate of nasal colonization with Staphylococcus species Journal of Clinical and Diagnostic Research. 2007 Dec; 1(6):494-499.
[22]
Shanmugam J., Gopal R., Kumar S.S. (2009). The prevalence, antibiogram and characterisation of staphylococcus aureus including MRSA among the healthy staff, medical students and patients from Sri Manakula Vinayagar Medical College and Hospital (SMVMCH), Puducherry. DSTE project report (Government of Puducherry).
[23]
Tortajada, G.M., Ferre, F.A., Tallon, G.M. and Garcia, M.E. (2008). “Hypersensitivity to clavulanic acid in children”. Allergol Immunopathol (Madr). 36(5):308-10.
[24]
Tou, P., Montobbio, G., Massone, G. and Matero, A. (1995). Nosocomial infection caused by multi-resistant staphylococci in a neonatal and paedistric intensive care unit. Paedistr. Med. chir. 17(1):117-122.
