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Isolation, Structural Characterization and Antioxidant Activities of Polysaccharide from Ganoderma lucidum (Higher Basidiomycetes)
Current Issue
Volume 3, 2015
Issue 5 (October)
Pages: 168-175   |   Vol. 3, No. 5, October 2015   |   Follow on         
Paper in PDF Downloads: 49   Since Sep. 8, 2015 Views: 2121   Since Sep. 8, 2015
Authors
[1]
Kannan Mohan, PG and Research Department of Zoology, Sri Vasavi College, Erode, India.
[2]
Muthusamy Padmanaban, PG and Research Department of Zoology, Sri Vasavi College, Erode, India.
[3]
Venkatachalam Uthayakumar, Departmentof Zoology, School of Life Sciences, Bharathiar University, Coimbatore, India.
Abstract
To study the isolation, structural characterization and antioxidant activities of polysaccharide from the medicinal mushroom G.lucidum. In this study structural characterization of Ganoderma lucidum polysaccharides was conducted by Fourier Transform Infrared spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM) analyses. Antioxidant activities of Ganoderma lucidum polysaccharides was measured by 1,1–diphenyl–2–icryl-hydrazyl (DPPH-), Hydroxyl radical scavenging assay (HO-), superoxide anion (O-) radical scavenging assay and Ferrous ion (Fe2+) chelating assay. Scavenging effect of G. lucidum polysaccharides and ascorbic acid on the DPPH radical scavenging, Hydroxyl radical scavenging, superoxide anion radical scavenging and Ferrous ion (Fe2+) chelating radical scavenging-dependently increased and was 30.45% and 85.40%, 35.70% and 90.50%, 49.68% and 90.10%, 32.69% and 80.26% at the dose of 4 mg/ml respectively. The FT-IR spectra revealed the general characteristic absorbance peaks of the GLP. The SEM image demonstrated surface features of the GLP. This study suggests that the Ganoderma lucidum polysaccharides could potentially be used as natural antioxidants.
Keywords
Ganoderma lucidum Polysaccharide, Isolation, Structural Characterization, Antioxidant Activities
Reference
[1]
Yuan JF, Zhang ZQ, Fan ZC, Yang JX. Antioxidant effects and cytotoxicity of three purified polysaccharides from Ligusticum chuanxiong Hort. Carbohydr Polym 2008; 74: 822-827.
[2]
Nandita S, Rajini PS. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem 2004; 85: 611-616.
[3]
Barros L, Ferreira MJ, Queiros B, Ferreira ICFR, Baptista P. Total phenols, ascorbic acid, b-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chem 2007; 103: 413-419.
[4]
Wasser SP, Weis AL. Therapeutic effects of substances occurring in higher basidiomycetes mushrooms: a modern perspective. Crit Rev of Immunol 1999; 19: 65-69.
[5]
Paterson RRM. Ganoderma – A therapeutic fungal biofactory. Phytochemistry 2006; 67: 1985-2001.
[6]
Liu YW, Gao JL, Gusn J, Qian ZM, Feng K, Li SP, et al. Evaluation antiproliferative activities and action mechanisms of extracts from two species of Ganoderma on tumor cell lines. J Agric Food Chem 2009; 57: 3087-3093.
[7]
Xu Z, Chen X, Zhong Z, Chen L, Wang Y. Ganoderma lucidum polysaccharides: Immunomodulation and potential anti-tumor activities. Am J Chin Med 2011; 39: 15-27.
[8]
Seo HW, Hung TM, Na M, Jung HJ, Kim JC, Choi JS, et al. Steroids and triterpenes from the fruit bodies of Ganoderma lucidum and their anti-complement activity. Arch Pharmacal Res 2009; 32: 1573-1579.
[9]
Dudhgaonkar S, Thyagarajan A, Sliva, D. Suppression of the inflammatory response by triterpenes isolated from the mushroom Ganoderma lucidum. Int Immunopharmacol 2009; 9: 1272-1280.
[10]
Sato N, Zhang Q, Ma CM, Hattori M. Anti-human immunodeficiency virus-1 protease activity of new lanostane-type triterpenoids from Ganoderma sinense. Chem Pharm Bull 2009; 57: 1076-1080.
[11]
Chen Y, Xie MY, Nie SP, Li C, Wang YX. Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum. Food Chem 2008; 107:231–241.
[12]
Weng Y, Xiang L, Matsuura A, Zhang Y, Huang Q, Qi J. Ganodermasides A and B, two novel anti-aging ergosterols from spores of a medicinal mushroom Ganoderma lucidum on yeast via UTH1 gene. Bioorg Med Chem 2010; 18: 999-1002.
[13]
Seto SW, Lam TY, Tam HL, Au ALS, Chan SW, Wu JH, Yu PHF, Leung GPH, Ngai SM, Yeung JHK, Leung PS, Lee SMY, Kwan YW, et al. Novel hypoglycemic effects of Ganoderma lucidum water-extract in obese/diabetic (+db/ +db) mice. Phytomedicine 2009; 16: 426-436.
[14]
Kim JH, Lee DHY, Lee SH, Choi SY, Lee JS. Effect of Ganoderma lucidum on the Quality and Functionality of Korean Traditional Rice Wine Yakju. J Biosci Bioeng 2004; 97: 24–28.
[15]
Despotovic S, Leskosek CI, Anita K, Nedovic V, Nikicevic N, Niksic M, et al. Effects of Ganoderma lucidum extracts on beer flavour. In: Proc. of 5th International Congress on Food Technology, Proceedings, Thessaloniki, Greece, 2007; pp. 370-375.
[16]
Hua KF, Hsu HY, Chao LK, Chen ST, Yang WB, Hua J, et al. Ganoderma lucidum polysaccharides enhance CD14 endocytosis of LPS and promote TLR4 signal transduction of cytokine expression. J Cell Physiol 2007; 212: 537-550.
[17]
Ji Z, Tang Q, Zhang J, Yang Y, Jia W, Pan Y, et al. Immunomodulation of RAW264.7 macrophages by GLIS, a proteo polysaccharides from Ganoderma lucidum. J Ethno Pharmacol 2007; 112: 445-450.
[18]
Xu J, Liu W, Yao WB, Pang XB, Yin DK, Gao XD, et al. Carboxymethylation of polysaccharides extracted from Ganoderma lucidum enhances its antioxidant activities in vitro. Carbohydr Polym 2009; 78: 227-234.
[19]
Mau JL, Lin HC, Chen CC. Antioxidant properties of several medicinal mushrooms. J Agric Food Chem 2002; 50: 6072-6077.
[20]
Lee JM, Kwon H, Jeong H. Inhibition of lipid peroxidation and oxidative DNA damage by Ganoderma lucidum. Phytother Res 2001; 15: 245–249.
[21]
Shi YL, James AE, Benzie IF, Buswell JA. Mushroom-derived preparations in the prevention of H2O2-induced oxidative damage to cellular DNA. Teratog Carcinog Mutagen 2002;22: 103–111.
[22]
Wachtel-Galor S, Choi SW, Benzie IFF. Effect of Ganoderma lucidum on human DNA is dose dependent and mediated by hydrogen peroxide. Redox Rep 2005; 10: 145–149.
[23]
Yuen JW, Gohel MD. The dual roles of Ganoderma antioxidants on urothelial cell DNA under carcinogenic attack. J Ethnopharmacol 2008; 118: 324–330.
[24]
Saltarelli R, Ceccaroli P, Iotti M. Biochemical characterization and antioxidant activity of mycelium of Ganoderma lucidum from Central Italy. Food Chem 2009; 116: 143–151.
[25]
Wu Y, Wang D. A new class of natural glycopeptides with sugar moiety-dependent antioxidant activities derived from Ganoderma lucidum fruiting bodies. J Proteome Res 2009; 8: 436- 442.
[26]
Ooi VE, Liu F. Immunomodulation and anti-cancer activity of polysaccharide-protein complexes. Curr Med Chem 2000; 7: 715-729.
[27]
Sheena MA, Ajith Janardhanan K. Prevention of nephrotoxicity induced by the anticancer drug Cisplatin, using Ganoderma lucidum, a medicinal mushroom occurring in South India. Current Sci 2003; 85: 478-482.
[28]
Jia J, Zhang X, Hu YS, Wu Y, Wang QZ, Li NN, Guo QC, Dong XC, et al. Evaluation of in vivo antioxidant activities of Ganoderma lucidum polysaccharides in STZ-diabetic rats. Food Chem 2009; 115: 32-36.
[29]
Mizuno T. Development of an antitumor biological response modifier from Phellinus linteus Teng. (Review). Int J Med Mushrooms 2000; 2: 21-33.
[30]
Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthone on the auto oxidation of soybean in cylcodextrin emulsion. J Agric Food Chem1992; 40: 945-948.
[31]
Eklund PC, Langvik OK, Warna JP, Salmi TO, Willfor SM, Sjoholm RE. Chemical studies on antioxidant mechanisms and free radical scavenging properties of lignans. Org Bimol Chem 2005; 21: 3336–3347.
[32]
Kordail S, Cakir A, Mavi A, Kilic H, Yildirin A. Screening of chemical composition and antifungal and antioxidant activities of the essential oil from three Turkish Artemisia species. J Agric Food chem2005; 53: 1408- 1416.
[33]
Nishikimi M, Rao NA, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochem Biophys Res Commun 1972; 46: 849-864.
[34]
Kuda T, Hishi T, Maekawa S. Antioxidant properties of dried product of ‘haba-nori’, an edible brown alga, Petalonia binghamiae (J. Agaradh) Vinogradova. Food Chem 2006; 98: 545–550.
[35]
Vissotto LC, Rodrigues E, Chisté RC, Benassi MT, Mercadante AZ. Correlation, by multivariate statistical analysis, between the scavenging capacity against reactive oxygen species and the bioactive compounds from frozen fruit pulps. Ciência e Tecnologia de Alimentos, 33 (supl. 1), 2013: pp. 57-65.
[36]
Dinis TCP, Madeira VMC, Almeida LM Action of phenolic derivates aceto amino phen, salycilate and 5-aminosalycilate as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 1994; 315: 161–169.
[37]
Monsoor MA, Kalapathy U, Proctor A. Determination of polygalacturonic acid content in pectin extracts by diffuse reflectance Fourier transform infrared spectroscopy. Food Chem 2001; 74: 233–238.
[38]
Su MS, Shyu YT, Chien PJ. Antioxidant activities of citrus herbal product extracts. Food Chem 2008; 111: 892–896.
[39]
Gülçinİ, Oktay M, Kireççi E, Küfrevioğlu Öİ. Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extracts. Food Chem2003; 83: 371−382.
[40]
Yuan YV, Bone DE, Carrington MF. Antioxidant activity of dulse (Palmaria palmata) extract evaluated in vitro. Food Chem2005; 91: 485-494.
[41]
Luo A, Fan, Y, Luo A. In vitro free radicals scavenging activities of polysaccharide from Polygonum Multiflorum Thunb. J Med Plants Res 2011; 5: 966–972.
[42]
Kozarski M, Klaus A, Niksˇic´ M, Vrvic´ MM, Todorovic´ N, Jakovljevic D, et al. Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor. J Food Comp Anal 2012; 26: 144–153.
[43]
Casu SG, Cifonelli AJ, Perlin AS. Infra-red spectra of glycosaminoglycans in deuterium oxide and deuterium chloride solution: Quantitative evaluation of uronic acid and acetamido deoxyhexose moieties. Carbohydr Res 1978; 63: 13–27.
[44]
Lo TCT, Chang CA, Chiu KH, Tsay PK, Jen J. Correlation evaluation of antioxidant properties on the monosaccharide components and glycosyl linkages of polysaccharide with different measuring methods. Carbohydr Polym 2011; 86: 320–327.
[45]
Naik GH, Priyadarsini KI, Satav JG, Banavalikar MM, Sohoni DP, Biyani MK, et al. Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine. Phytochemistry 2003; 63: 97–104.
[46]
Liu J, Jia L, Kan J, Jin CH. In vitro and in vivo antioxidant activity of Ethanolic extract of white button mushroom (Agaricus bisporus). Food Chem Toxicol 2013; 51: 310–316.
[47]
Oke F, Aslim B, Ozturk S, Altundag S. Essential oil composition, antimicrobial and antioxidant activities of Satureja cuneifolia Ten. Food Chem 2009; 112: 874-879.
[48]
Lin HC. Evaluation of taste quality and antioxidant properties of edible and medicinal mushrooms. Master’s thesis, National Chung-nHsing University, Taichung, Taiwan, 1999.
[49]
Gao Y, Tang W, Gao H, Chan E, Lan J, Li X, Zhou S, et al. Antimicrobial activity of the medicinal mushroom Ganoderma. Food Rev Int 2005; 21: 211–222.
[50]
Liu F, Ooi VE, Chang ST. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci 1997; 60: 763–771.
[51]
Zou Y, Qian ZJ, Lee SH, Li Y, Kim MM, Kim SK, et al. Antioxidant effects of phlorotannins isolated from ishige okamurae in free radical mediated oxidative systems. J Agric Food Chem 2008; 56: 7001-7009.
[52]
Yamaguchi R, Tatsumi MA, Kato K, Yoshimitsu U. Effect of metal salts and fructose on the autoxidation of methyl linoleate in emulsions. Agric Biol Chem 1988; 52: 849-850.
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