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Different Assessments of the Effect of Drying Rates on Recalcitrant Seed Material
Current Issue
Volume 3, 2015
Issue 3 (June)
Pages: 75-79   |   Vol. 3, No. 3, June 2015   |   Follow on         
Paper in PDF Downloads: 19   Since Aug. 28, 2015 Views: 1209   Since Aug. 28, 2015
Authors
[1]
Tobias M. Ntuli, Plant Germplasm Conservation Research, School of Biological & Conservation Sciences, University of KwaZulu-Natal (Westville Campus), Durban, South Africa; Department of Life and Consumer Sciences, University of South Africa, Florida, South Africa.
[2]
Patricia Berjak, Plant Germplasm Conservation Research, School of Biological & Conservation Sciences, University of KwaZulu-Natal (Westville Campus), Durban, South Africa.
[3]
N. W. Pammenter, Plant Germplasm Conservation Research, School of Biological & Conservation Sciences, University of KwaZulu-Natal (Westville Campus), Durban, South Africa.
Abstract
The results of the germination and tetrazolium (TZ) tests of axes of Pisum sativum, Quercus robur and Trichilia dregeana were in agreement during both drying and wet storage. The TZ test overestimated viability of Avicennia marina, Trichilia emetica and Strychnos madagascariensis axes in comparison to the germination test during dehydration. Thus, the germination test is a method of choice during desiccation and the TZ test may be a better indicator of viability during hydrated storage than drying. The survival of axes of Q. robur during slow dehydration and moist storage was unexpectedly poor possibly as a result of an unfavourable temperature during treatments. Hence, rapid desiccation is recommended for determinations of minimum ‘critical water contents’. The relationship between electrolyte leakage and water content during drying and wet storage of axes of T. dregeana harvested in 2001 and S. madagascariensis showed a ‘classic pattern’ as dehydration and hydrated storage proceeded. Consequently, the conductivity test is a poor measure of the ‘critical water contents’ but may be an indicator of differences in vigour among harvests of the same species along with the germination and TZ tests during slow desiccation. Less leakage occurred during rapid than slow drying in all species. Desiccation-sensitive seeds can be divided into three categories on the basis of the predominant mechanism of loss of viability during dehydration: minimally, moderately and highly desiccation-sensitive in which desiccation, metabolic and physical damage predominate, respectively. Irrespective of the mode of dying, the effect of rate drying of was always apparent.
Keywords
Conductivity, Desiccation, Drying Rate, Recalcitrant, Storage, Viability
Reference
[1]
Berjak P., Dini, M. and Pammenter, N.W. (1984). Possible mechanisms underlying the differing dehydration responses in recalcitrant and orthodox seeds: desiccation associated subcellular changes in propagules of Avicennia marina. Seed Science and Technology 12, 365-384.
[2]
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[3]
Ntuli, T. M. and Pammenter, N. W. (2011). Dehydration kinetics of embryonic axes from desiccation-sensitive seeds: an assessment of descriptive models. Journal of Integrative Plant Biology 51, 1002-1007.
[4]
Ntuli, T. M., Berjak, P and Pammenter, N. W. (1997). Effects of temperature on the desiccation responses of seeds of Zizania palustris. Seed Science Research 7, 145-160.
[5]
Ntuli, T. M., Berjak, P and Pammenter, N. W. (2014). Tissue diversity in respiratory metabolism and free radical processes in embryonic axes of the white mangrove (Avicennia marina) during drying and wet storage. African Journal of Biotechnology 13, 1813-1823.
[6]
Ntuli, T. M., Pammenter, N. W. and Berjak, P. (2013). Increasing the rate of drying reduces metabolic imbalance, lipid peroxidation and critical water content in radicles of garden pea. Biological Research 46, 121-130.
[7]
Pammenter, N.W., Berjak, P., Wesley-Smith, J. and Vander Willigen, C. (2002). Experimental aspects of drying and recovery. In: Black, M. and Pritchard, H.W. (eds) Desiccation and Survival in Plants, CABI, Wallingford, UK. Chap 3, pp 93-110.
[8]
Pammenter, N.W., Vertucci, C.W. and Berjak, P. (1991). Homoiohydrous (recalcitrant) seeds: dehydration, the state of water and viability characteristics in Landolphia kirkii. Plant Physiology 96, 1093-1098.
[9]
Peran, R, Pammenter, N.W., Naicker, J. and Berjak, P. (2004). The influence of rehydration technique on the response of recalcitrant seed embryos to desiccation. Seed Science Research 14, 179-184.
[10]
Walters, C., Pammenter, N. W., Berjak, P. and Crane, J. (2001). Desiccation damage, accelerated aging and respiration. Seed Science Research 11, 135-148.
[11]
Wesley-Smith, J., Vertucci, C.W., Berjak, P., Pammenter, N.W. and Crane, J. (1992). Cryopreservation of recalcitrant axes of Camellia sinensis in relation to dehydration, freezing rate and the thermal properties of tissue water. Journal of Plant Physiology 140, 596-604.
[12]
Wesley-Smith, J., Walters, C., Pammenter, N.W. and Berjak, P. (2001). Interaction of water content, rapid (non equilibrium) cooling to –196 oC, and survival of embryonic axes of Aesculus hippocastanum L. seeds. Cryobiology 42, 196-206.
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