Micropropagation of Chlorophytum borivilianum: In vitro Clonal Fidelity Test and Antioxidant Enzymatic Study
The regeneration of Chlorophytum borivilianum is generally through tuberous roots, which have become scarce in nature. Seed germination is only 14-16%, thus an in vitro method for conservation and multiplication of this species could be valuable. In vitro clonal propagation of safed musli has been achieved on MS medium augmented with auxins: 2, 4-Dicholorophenoxyacetic acid (2, 4-D), 1-Naphthaleneacetic acid (NAA) and cytokinins: Benzyladenine (BA), Kinetin (Kn). There was a 90% shoot multiplication response obtained at MS + 4.4 µM BA. Isolated shoots were transferred on ½ MS medium supplemented with 2.4 µM IBA (indole-3-acetic acid) which gave 90% rooting response, were further acclimatized and hardened to garden successfully. In vitro regenerated plants have been tested for clonal fidelity using RAPD markers. It is first report on biochemical changes of catalase, peroxidase, super oxide dismutase, protein and chlorophyll content during in vitro regeneration in C. borivilianum.
Chlorophytum borivilianum, RAPD, Catalase, Genetic Fidelity, Superoxide Dismutase
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