The Analgesic Effect of Tanacetum parthenium Extract in Formalin, Acid Acetic and Hot Plate Test in Mice
Tanacetum parthenium belongs to the Asteraceae family. This medicinal plant and other species have been widely used around the world since ancient times. It has been utilized to treat many diseases such as abdominal pain, indigestion and diabetes. In this experimental study, the analgesic effects of Tanacetum parthenium by formalin, hot plate and acetic acid test were evaluated in mice. In each test, the groups included distilled water (control), the extract with doses of 10, 20, 30, and 40mg/kg, ibuprofen 100 mg /kg, morphine 0.5 mg/kg, naloxane of 0.5 mg/kg and naloxane of 0.5 mg/kg and 10 and 40 mg/kg which was found to be the most effective dose of extract. Animals were injected with 0.9% acetic acid for visceral pain induction. 15 minutes after each injection, anti-nociceptive effects were recorded by counting the number of writhes during 30 minutes. In formalin test licking the hind-limb or jumping was recorded during 30 minutes. Mice were placed on hot plate with constant temperature of 48°C (5-minute intervals) for an hour. Results: The findings of this study showed that the ethanolic extract of Tanacetum parthenium had an analgesic effect (P<0.05). Injection of Naloxone blocked the analgesic effects of extract in formalin (61±3.15 to 111.5±56.4) and acid acetic test (6.2±3.01 to 19.6±5.1).Conclusion: This study verified the anti-nociceptive properties of Tanacetum parthenium compared to the control group and the anti-nociceptive activity of Tanacetum parthenium extract is due to interaction with opioid system. However, further studies are necessary to find the mechanisms effect of Tanacetum parthenium on pain.
Morphine, Opioid, Visceral Pain, Tanacetum parthenium
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